If you like the way iTunes has shuffled the play order of one of your play lists, you can use the "Copy to Play Order" shortcut menu command to make the shuffled play order the permanent play order. To use this command, Control-click one of the songs in the playlist, and choose "Copy to Play Order" from the shortcut menu. Tip: These steps also apply to a playlist you have reordered directly (by clicking on one of the column headers like Artist or Album). Important: This command will only appear if a playlist has been shuffled or reordered. Figure 1
Source >>